Course Description
n This course focuses on all aspects and steps of an analytical method to determine and separate biomolecules (endogenous compounds, environmental pollution, drugs etc.) in various biological matrices (like serum, urine, cell culture) and environmental samples (water) , The first part of this course is dedicated to biomolecules (amino acids, peptides, proteins, nucleic acids and classical analytical chemistry, limitations and bioanalytical chemistry) , The second part deals with chromatography (principle, basic theory, application of liquid chromatography for bioanalysis: reversed phase, ion exchange, affinity and size exclusion) , The third chapter is reserved to electrophoresis (principle and theory, gel electrophoresis and capillary electrophoresis) , The fourth part studied mass spectrometry (principle, MALDI-TOF/MS, ESI/MS and applications) , The fifth chapter is dedicated to molecular recognition (bioassays, antibodies, antigens, enzyme immunoassays, biosensors, DNA-arrays, identification and pyrosequencing) , The sixth chapter deals with nucleic acids (extraction and isolation and protein sequencing (sequencing strategy, N-terminal analysis, C-terminal analysis, separation and molecular weight determination).
Course ID: Chem 662
| Credit hours | Theory | Practical | Laboratory | Lecture | Studio | Contact hours | Pre-requisite | 3 | - | - |
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