التحليل الكيميائي الحديث

Course Description

 Supercritical fluid chromatography , Introduction, History of SFC, Basic Principles in SFC, Parameter optimization,
Instrument requirements, Current Examples, Chiral separations, Polymer separations, High throughput screening of pharmaceuticals , Electromigration methods , origins, principles, and applications
Introduction, Fundamentals of CE, Electrophoretic mobility, Electroosmotic mobility , Electrophoretic migration, Efficiency, Selectivity and resolution, Instrumentation
, Sample injection, Detection, Sensitivity enhancement, Time-corrected (normalized) areas, Thermostatting, Methods and Modes in CE, Moving boundary CE, Steady-state capillary electrophoresis, Zone capillary electrophoresis .
Hyphenated methods:
Introduction, Mass Spectrometry, Molecular weight vs. relative-molecular mass, Monoisotopic vs. chemical (average) relative molecular mass, Polyisotopic elements and isotope patterns, High resolution measurements vs. accurate mass measurements, Ionization methods, Gas Chromatography-Mass Spectrometry, Historical perspective, GC-MS interfaces, Available ionization methods, Applicability to “ chemical space”, Identification of compounds, Mass spectral libraries, The GC-MS data mode), Full ( Repetitive) scan data collection vs. selected ion recording, GC-MS Example- residual solvents analysis, Liquid Chromatography-Mass Spectrometry, Historical perspective, Evolution of LC-MS interfaces, LC-MS compatible mobile phases, LC-MS accurate mass measurements An LC-MS example. Nuclear Magnetic Resonance Spectroscopy, Background, Liquid chromatography-nuclear magnetic resonance, Historical perspective, Basic instrumentation, Modes of operation, Continuous flow, Stop-flow, Peak parking, Peak trapping, Liquid chromatography-nuclear magnetic, resonance-mass spectroscopy, Recent advances, Conclusions.
Radiochemical Method, Radioactive Nuclide Instrumentation, Neutron Activation Method, Isotope Dilution Method.
Automated Methods of Analysis, Overview, Flow Injection Analysis, Microfluidics, Discrete Automatic Systems.

Course ID: Chem 663